Zeedman
Garden Master
I agree that after moisture & temperature, low oxygen is the next most important factor in long term seed storage. I've utilized that philosophy by storing seed in zippered freezer bags, and rolling them tightly to expel as much air as possible before closing. Provided that the seed has been dried to a low moisture content, this will extend seed life beyond the often-posted limits - even stored at room temperature. My original reference for this was a Spanish study which I can no longer locate, but the reference below (while dated) has similar information, and is still useful for serious seed savers:The best way to store seeds is with less oxygen getting to the seed. Colder and dryer the storage environorment the better. Oxygen oxidizes not only the seed coats and continues to darken them with time, but oxygen also oxidizes the amino acids in the seed that is reponsible for getting the embryo plant inside the seed to come out of it's dormancy when the temperature is high enough and the seed has taken in enough water. The oxidization of these amino acids is why old seed when old enough and enough of the amino acids have oxidized the seed no longer comes to life. Look at what they do at the World Seed Bank in Svalbard. They put seeds in special laminated seed packets so they won't absorb any moisture with all the air vacuumed out of the packets. Storage temperatures are what -18 C or
-.4 F. The thing about keeping seed cold and dry is the viablility will be lengthened the more even the temperature is maintained. At Svalbard the sourrounding mountain will keep tempertures very even. They figure that seed stored there will remain viable for 500 years. I'm not going to live 500 years but I'll settle on my freezers. I have already acquired bean seed that was stored in a freezer for 17 years and my germination when planting was 100%. The best freezers to store seed is one that is not frost free. The walls keep warming up to eliminate frost build up and I have been told that will affect the seed stored in a frost free freezer. So in a nutshell. For long seed viability. Colder, dryer and the less oxygen the better. You know those silica gel tabs that absorb moisture. Well there also exists little tabs like that you can place inside little ziplocs and they will absorb the oxygen that is in any air inside your baggie.
Then with your seed frozen you want to get at it sometime to grow. Allow frozen seed to warm naturally to the out of freezer enviornment. You don't just want to open a container of frozen seed until it has equalized with the tempearture in your room. It harms viability when suddenly exposing frozen seed to the air.
Priciples and Practices of Seed Storage
Many years ago, I acquired a large can of commercial seed, for water spinach. I didn't open that can for 5-6 years, until I had used up the seed I already had. When I finally opened that can, germination was very high. The label on the can said that the seeds were packed in nitrogen, so obviously the lack of oxygen had not harmed them. This was one kilo of seed, so I divided it into 3 jars; one that I am currently using, one in the refrigerator, and one in the freezer... that seed should last as long as I am able to garden.